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ORIGINAL ARTICLE
Year : 2008  |  Volume : 8  |  Issue : 4  |  Page : 207-212

A comparative in-vitro microbiological study to evaluate the penetration by Candida albicans of different heat cure acrylic resins after denture brush abrasion


1 Department of Prosthodontics, M.S. Ramaiah Dental College, Bangalore, India
2 Department of Microbiology, M.S. Ramaiah Dental College, Bangalore, India

Correspondence Address:
Amit Dua
IV-!/76, Gopinath Bazaar, Delhi Cantt. New Delhi-110 010
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-4052.49184

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Context: Failure to maintain adequate hygiene and surface roughness of the denture have been shown to be associated with a high level of oral Candida colonization. Aims: To evaluate and compare the short term adhesion and long term penetration of Candida albicans into different types of heat cure acrylic materials, with and without denture brush abrasion and to compare the adherence and penetration among these materials. Material and Methods: Three different heat cure denture base materials viz., DPI high impact, DPI conventional and DPI tooth colored were used in the study. Thirty-one test samples were fabricated for each. Nineteen test samples were subjected to brushing. All of them were incubated and examined for adherence using fluorescent microscopy at time intervals of one hour and six weeks, and for penetration after six weeks of incubation. Statistical Analysis Used: Analysis of variance (ANOVA) was used to compare the mean Candida albicans cells of the various denture base materials at one hour adherence, six weeks adherence and six weeks penetration. Results: There was less surface adherence and penetration in high impact denture material as compared to conventional and tooth colored ones. Conclusions: High impact acrylic resin shows less adherence and penetration of Candidial cells compared to the conventional and tooth colored acrylic resins.Brushing alone does not completely eradicate all the Candidial cells present.Samples brushed initially show more Candidial cells as compared to unbrushed ones.


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